Difference between revisions of "Part:BBa K3027002"

 
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Nuclease_yqcG (BBa_K3027002) coding sequence from bacteria Bacillus subtilis strain 168 (1596 nucleotides):
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        1 atgaaagta tttgaagcca aaaccttgct caccgaggct gaaaagcgag
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      50 cacaggaata caaagattta aaaagtaaaa tggtcaaatt aaagaaagcg ttcaaagctg
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      110 ttgcagattt agatgatagc gagttttcag gcaaaggcgc taataacatt aaatcatttt
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      170 atgaagatca agctggcatt gctgaccaat ggattgattt aattgagatg aaaatttctt
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      230 tcttaacgag tattcctggt tttttagaag atgccaactt atcagatgcc tacatagaag
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      290 aaaccttttt agcgcatgag cttgctaacg cctacaccaa atcaaaatcc attatgtctg
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      350 aacagaaaaa agccatgaaa gatatattaa atgatatcaa cgatatactg cctttggatc
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      410 tgttctcgac agaaactttt aaaaatgaac tttcgtctgc cgaaaagaaa cgaaaagaag
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      470 ccattgagaa aatggatgaa gttgaccaaa acttaacatc agaatacgga ctatcagaag
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      530 caaatgaaca aatgattcaa gctgattatc aagctttaat gaatgcgaca gcaaaaggca
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      590 aaagcgcctc tcctattcac tataatgcga aggcttatag agacagtgag attcacaaga
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      650 tgactgagga tgtaaagaaa caatcaacag actacatctc ttttaaggac caacaggcgg
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      710 aacaaagaag aattgcaaaa gaacaagaag aactggcgaa cagaccatgg tatgaaaagt
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      770 cttgggatgc tgtttgtaat tttacaggag aggtgtctgg atattatgat tataaaagag
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      830 ccgctgatgg ggttgatcct gttaccggcg aaaagctaac tgctggacaa cgggtcgctg
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      890 ccggtgcaat ggctgcggca ggatacatcc ctatcgtagg atgggcaggt aaattagcta
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      950 aaggtggaaa agctgtttat tcaacaagta aagcattgta tagagcagac aaagctctcg
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    1010 atgtatataa aacacctaag acattccatg cccttcaaaa ctccagcaaa ggactttatg
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    1070 gacttgcttc tgcaaatggt tttagtgaag ctataacagg acgcgatatg ttcgggaata
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    1130 aagtctccaa agagaggcaa gaacagagcc ttagtggtgc aatggcaatg ttggttcctt
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    1190 tcggagctcg cggaattaac aaaaagctta atgcaaaatc aagttctcga gttagcgaag
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    1250 catcgactaa caccagcaaa aaacctaaag ttcctaaaac gtacaaaaga ccaacttatt
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    1310 tccgtaaagg cgttagagat aaggtttggg aaaatgctaa ggattcaacc ggatctgtta
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    1370 aagacccgtt aacaaaacaa gtaatgaaaa aagatgaacc atgggatatg ggtcataaac
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    1430 caggttatga atttagaaaa catcaacaaa gtgcgatgga aagaaatatt agtcgaaagc
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    1490 aatttttaga tgaacataat aatccagacc actatcaacc cgaattacct tcctccaata
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    1550 ggagtcataa aggggaagat atgacggatg attactttgg agattaa
  
__NOTOC__
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<partinfo>BBa_K3027002 short</partinfo>
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CDS of the YqcG nuclease.
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Nuclease_gp3 sequence (531 amino acids):
  
<!-- Add more about the biology of this part here
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Met Lys Val Phe Glu Ala Lys Thr Leu Leu Thr Glu Ala Glu Lys Arg Ala Gln Glu Tyr Lys Asp Leu Lys Ser Lys Met Val Lys Leu Lys Lys Ala Phe Lys Ala Val Ala Asp Leu Asp Asp Ser Glu Phe Ser Gly Lys Gly Ala Asn Asn Ile Lys Ser Phe Tyr Glu Asp Gln Ala Gly Ile Ala Asp Gln Trp Ile Asp Leu Ile Glu Met Lys Ile Ser Phe Leu Thr Ser Ile Pro Gly Phe Leu Glu Asp Ala Asn Leu Ser Asp Ala Tyr Ile Glu Glu Thr Phe Leu Ala His Glu Leu Ala Asn Ala Tyr Thr Lys Ser Lys Ser Ile Met Ser Glu Gln Lys Lys Ala Met Lys Asp Ile Leu Asn Asp Ile Asn Asp Ile Leu Pro Leu Asp Leu Phe Ser Thr Glu Thr Phe Lys Asn Glu Leu Ser Ser Ala Glu Lys Lys Arg Lys Glu Ala Ile Glu Lys Met Asp Glu Val Asp Gln Asn Leu Thr Ser Glu Tyr Gly Leu Ser Glu Ala Asn Glu Gln Met Ile Gln Ala Asp Tyr Gln Ala Leu Met Asn Ala Thr Ala Lys Gly Lys Ser Ala Ser Pro Ile His Tyr Asn Ala Lys Ala Tyr Arg Asp Ser Glu Ile His Lys Met Thr Glu Asp Val Lys Lys Gln Ser Thr Asp Tyr Ile Ser Phe Lys Asp Gln Gln Ala Glu Gln Arg Arg Ile Ala Lys Glu Gln Glu Glu Leu Ala Asn Arg Pro Trp Tyr Glu Lys Ser Trp Asp Ala Val Cys Asn Phe Thr Gly Glu Val Ser Gly Tyr Tyr Asp Tyr Lys Arg Ala Ala Asp Gly Val Asp Pro Val Thr Gly Glu Lys Leu Thr Ala Gly Gln Arg Val Ala Ala Gly Ala Met Ala Ala Ala Gly Tyr Ile Pro Ile Val Gly Trp Ala Gly Lys Leu Ala Lys Gly Gly Lys Ala Val Tyr Ser Thr Ser Lys Ala Leu Tyr Arg Ala Asp Lys Ala Leu Asp Val Tyr Lys Thr Pro Lys Thr Phe His Ala Leu Gln Asn Ser Ser Lys Gly Leu Tyr Gly Leu Ala Ser Ala Asn Gly Phe Ser Glu Ala Ile Thr Gly Arg Asp Met Phe Gly Asn Lys Val Ser Lys Glu Arg Gln Glu Gln Ser Leu Ser Gly Ala Met Ala Met Leu Val Pro Phe Gly Ala Arg Gly Ile Asn Lys Lys Leu Asn Ala Lys Ser Ser Ser Arg Val Ser Glu Ala Ser Thr Asn Thr Ser Lys Lys Pro Lys Val Pro Lys Thr Tyr Lys Arg Pro Thr Tyr Phe Arg Lys Gly Val Arg Asp Lys Val Trp Glu Asn Ala Lys Asp Ser Thr Gly Ser Val Lys Asp Pro Leu Thr Lys Gln Val Met Lys Lys Asp Glu Pro Trp Asp Met Gly His Lys Pro Gly Tyr Glu Phe Arg Lys His Gln Gln Ser Ala Met Glu Arg Asn Ile Ser Arg Lys Gln Phe Leu Asp Glu His Asn Asn Pro Asp His Tyr Gln Pro Glu Leu Pro Ser Ser Asn Arg Ser His Lys Gly Glu Asp Met Thr Asp Asp Tyr Phe Gly Asp
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3027002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3027002 parameters</partinfo>
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<!-- -->
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Revision as of 16:02, 13 October 2019

Nuclease_yqcG (BBa_K3027002) coding sequence from bacteria Bacillus subtilis strain 168 (1596 nucleotides):

       1 atgaaagta tttgaagcca aaaccttgct caccgaggct gaaaagcgag
      50 cacaggaata caaagattta aaaagtaaaa tggtcaaatt aaagaaagcg ttcaaagctg
     110 ttgcagattt agatgatagc gagttttcag gcaaaggcgc taataacatt aaatcatttt
     170 atgaagatca agctggcatt gctgaccaat ggattgattt aattgagatg aaaatttctt
     230 tcttaacgag tattcctggt tttttagaag atgccaactt atcagatgcc tacatagaag
     290 aaaccttttt agcgcatgag cttgctaacg cctacaccaa atcaaaatcc attatgtctg
     350 aacagaaaaa agccatgaaa gatatattaa atgatatcaa cgatatactg cctttggatc
     410 tgttctcgac agaaactttt aaaaatgaac tttcgtctgc cgaaaagaaa cgaaaagaag
     470 ccattgagaa aatggatgaa gttgaccaaa acttaacatc agaatacgga ctatcagaag
     530 caaatgaaca aatgattcaa gctgattatc aagctttaat gaatgcgaca gcaaaaggca
     590 aaagcgcctc tcctattcac tataatgcga aggcttatag agacagtgag attcacaaga
     650 tgactgagga tgtaaagaaa caatcaacag actacatctc ttttaaggac caacaggcgg
     710 aacaaagaag aattgcaaaa gaacaagaag aactggcgaa cagaccatgg tatgaaaagt
     770 cttgggatgc tgtttgtaat tttacaggag aggtgtctgg atattatgat tataaaagag
     830 ccgctgatgg ggttgatcct gttaccggcg aaaagctaac tgctggacaa cgggtcgctg
     890 ccggtgcaat ggctgcggca ggatacatcc ctatcgtagg atgggcaggt aaattagcta
     950 aaggtggaaa agctgtttat tcaacaagta aagcattgta tagagcagac aaagctctcg
    1010 atgtatataa aacacctaag acattccatg cccttcaaaa ctccagcaaa ggactttatg
    1070 gacttgcttc tgcaaatggt tttagtgaag ctataacagg acgcgatatg ttcgggaata
    1130 aagtctccaa agagaggcaa gaacagagcc ttagtggtgc aatggcaatg ttggttcctt
    1190 tcggagctcg cggaattaac aaaaagctta atgcaaaatc aagttctcga gttagcgaag
    1250 catcgactaa caccagcaaa aaacctaaag ttcctaaaac gtacaaaaga ccaacttatt
    1310 tccgtaaagg cgttagagat aaggtttggg aaaatgctaa ggattcaacc ggatctgtta
    1370 aagacccgtt aacaaaacaa gtaatgaaaa aagatgaacc atgggatatg ggtcataaac
    1430 caggttatga atttagaaaa catcaacaaa gtgcgatgga aagaaatatt agtcgaaagc
    1490 aatttttaga tgaacataat aatccagacc actatcaacc cgaattacct tcctccaata
    1550 ggagtcataa aggggaagat atgacggatg attactttgg agattaa

Nuclease_gp3 sequence (531 amino acids):

Met Lys Val Phe Glu Ala Lys Thr Leu Leu Thr Glu Ala Glu Lys Arg Ala Gln Glu Tyr Lys Asp Leu Lys Ser Lys Met Val Lys Leu Lys Lys Ala Phe Lys Ala Val Ala Asp Leu Asp Asp Ser Glu Phe Ser Gly Lys Gly Ala Asn Asn Ile Lys Ser Phe Tyr Glu Asp Gln Ala Gly Ile Ala Asp Gln Trp Ile Asp Leu Ile Glu Met Lys Ile Ser Phe Leu Thr Ser Ile Pro Gly Phe Leu Glu Asp Ala Asn Leu Ser Asp Ala Tyr Ile Glu Glu Thr Phe Leu Ala His Glu Leu Ala Asn Ala Tyr Thr Lys Ser Lys Ser Ile Met Ser Glu Gln Lys Lys Ala Met Lys Asp Ile Leu Asn Asp Ile Asn Asp Ile Leu Pro Leu Asp Leu Phe Ser Thr Glu Thr Phe Lys Asn Glu Leu Ser Ser Ala Glu Lys Lys Arg Lys Glu Ala Ile Glu Lys Met Asp Glu Val Asp Gln Asn Leu Thr Ser Glu Tyr Gly Leu Ser Glu Ala Asn Glu Gln Met Ile Gln Ala Asp Tyr Gln Ala Leu Met Asn Ala Thr Ala Lys Gly Lys Ser Ala Ser Pro Ile His Tyr Asn Ala Lys Ala Tyr Arg Asp Ser Glu Ile His Lys Met Thr Glu Asp Val Lys Lys Gln Ser Thr Asp Tyr Ile Ser Phe Lys Asp Gln Gln Ala Glu Gln Arg Arg Ile Ala Lys Glu Gln Glu Glu Leu Ala Asn Arg Pro Trp Tyr Glu Lys Ser Trp Asp Ala Val Cys Asn Phe Thr Gly Glu Val Ser Gly Tyr Tyr Asp Tyr Lys Arg Ala Ala Asp Gly Val Asp Pro Val Thr Gly Glu Lys Leu Thr Ala Gly Gln Arg Val Ala Ala Gly Ala Met Ala Ala Ala Gly Tyr Ile Pro Ile Val Gly Trp Ala Gly Lys Leu Ala Lys Gly Gly Lys Ala Val Tyr Ser Thr Ser Lys Ala Leu Tyr Arg Ala Asp Lys Ala Leu Asp Val Tyr Lys Thr Pro Lys Thr Phe His Ala Leu Gln Asn Ser Ser Lys Gly Leu Tyr Gly Leu Ala Ser Ala Asn Gly Phe Ser Glu Ala Ile Thr Gly Arg Asp Met Phe Gly Asn Lys Val Ser Lys Glu Arg Gln Glu Gln Ser Leu Ser Gly Ala Met Ala Met Leu Val Pro Phe Gly Ala Arg Gly Ile Asn Lys Lys Leu Asn Ala Lys Ser Ser Ser Arg Val Ser Glu Ala Ser Thr Asn Thr Ser Lys Lys Pro Lys Val Pro Lys Thr Tyr Lys Arg Pro Thr Tyr Phe Arg Lys Gly Val Arg Asp Lys Val Trp Glu Asn Ala Lys Asp Ser Thr Gly Ser Val Lys Asp Pro Leu Thr Lys Gln Val Met Lys Lys Asp Glu Pro Trp Asp Met Gly His Lys Pro Gly Tyr Glu Phe Arg Lys His Gln Gln Ser Ala Met Glu Arg Asn Ile Ser Arg Lys Gln Phe Leu Asp Glu His Asn Asn Pro Asp His Tyr Gln Pro Glu Leu Pro Ser Ser Asn Arg Ser His Lys Gly Glu Asp Met Thr Asp Asp Tyr Phe Gly Asp