Difference between revisions of "Part:BBa K2923020:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
This part was created by Gibson assembly.
 
This part was created by Gibson assembly.
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===Source===
 
===Source===
  
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This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. The guanine aptazyme sequence is previously described by Felletti, M., Stifel, J., Wurmthaler, L.A., Geiger, S., and Hartig, J.S. (2016). Twister ribozymes as highly versatile expression platforms for artificial riboswitches. Nature Communications 7
+
This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. The guanine aptazyme sequence is previously described by Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.
  
 
===References===
 
===References===
 +
 +
Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46
 +
Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.

Latest revision as of 14:52, 13 October 2019


MS2-MS2-Guanine actif aptazyme-PP7-PP7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created by Gibson assembly.

Source

This present part is composed of two MS2 (from Escherichia phage MS2) and two PP7 (Pseudomonas phage PP7) stem-loops. The guanine aptazyme sequence is previously described by Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.

References

Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46 Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.