Difference between revisions of "Part:BBa K3076100:Design"
 
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<partinfo>BBa_K2578511 short</partinfo>
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<partinfo>BBa_K3076100 short</partinfo>
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<partinfo>BBa_K2578511 SequenceAndFeatures</partinfo>
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===Design Notes===
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The DNA sequence was modified to suit the synthesis by IDT while retaining the original amino acid sequence.
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<partinfo>BBa_K3076100 SequenceAndFeatures</partinfo>
  
  
 
===Source===
 
===Source===
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The <i>CgMT</i> sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for <i>E. coli</i> by the ThermoFisher GeneArt platform.
  
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NCBI
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===Design Notes===
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There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine.
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When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.
  
 
===References===
 
===References===
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[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064
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[1] NCBI gene ID 856450
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[2] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327
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[3] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.
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[4] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0
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[5] Brenes-Pomales, A., Lindegren, G., & Lindegren, C. C. (1955). Gene Control of Copper-Sensitivity in Saccharomyces. Nature, 176(4487), 841-842. doi:10.1038/176841a0
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[6] Adamo, G., Brocca, S., Passolunghi, S., Salvato, B., & Lotti, M. (2012). Laboratory evolution of copper tolerant yeast strains. Microbial Cell Factories, 11(1), 1. doi:10.1186/1475-2859-11-1
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[7] Berka, T., Shatzman, A., Zimmerman, J., Strickler, J., & Rosenberg, M. (1988). Efficient expression of the yeast metallothionein gene in Escherichia coli. Journal of Bacteriology, 170(1), 21-26. doi:10.1128/jb.170.1.21-26.1988
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Latest revision as of 13:41, 13 October 2019


Coding sequence of metallothioneins (MT) gene from Corynebacterium glutamicum (CgMT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The CgMT sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for E. coli by the ThermoFisher GeneArt platform.

Design Notes

There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine. When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.

References

[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064