Difference between revisions of "Part:BBa K3137006"

 
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<partinfo>BBa_K3137006 short</partinfo>
 
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DnaE is the alpha subunit of DNA polymerase III which catalyzes the polymerase activity of the holoenzyme complex.
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AbpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.
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Revision as of 13:33, 13 October 2019


abpAB

AbpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal BglII site found at 561
    Illegal BglII site found at 1621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 222
    Illegal EcoRI site found at 990
    Illegal PstI site found at 255
    Illegal PstI site found at 1647
    Illegal PstI site found at 2727
    Illegal NgoMIV site found at 3333
  • 1000
    COMPATIBLE WITH RFC[1000]