Difference between revisions of "Part:BBa K3076400:Design"
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===Design Notes===
 
===Design Notes===
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N/A
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The genomic sequence of cusA gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: 598714..601857). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry <html>(<a href="https://parts.igem.org/Part:BBa_B0015">BBa B0015</a>)</html> which is the most commonly used double terminator with high efficiency.
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+
  
  
 
===References===
 
===References===
−
The genomic sequence of cusA gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: 598714..601857). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.
 

Revision as of 13:01, 13 October 2019


dsDNA substrate with KanR gene for cusA knockout in E. coli by Lambda Red Recombineering


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The genomic sequence of cusA gene was extracted from NCBI (ACCESSION: NC_000913 REGION: 598714..601857). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa B0015) which is the most commonly used double terminator with high efficiency.


References