Difference between revisions of "Part:BBa K3113209"

Latest revision as of 12:44, 13 October 2019

pCAG_CD63_Ser161_6xHis_HiBit_P9SN

This plasmid-encoded for CD63 fused to the HiBiT peptide and P9SN. The 6xHis tag inserted into the large extracellular loop of CD63 is used to facilitate purification of successfully engineered exosomes. It is used to target fluc specifically into exosomes via coiled-coil fused RNA binding proteins. The HiBit peptide is used for detection via luminescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 12:38, 27 September 2019 (view source) Registry (Talk \| contribs)		Latest revision as of 12:44, 13 October 2019 (view source) Theresakeil (Talk \| contribs)
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−	This plasmid-encoded for CD63 fused to the HiBiT peptide and ~~CC9~~. The 6xHis tag inserted into the large extracellular loop of CD63 is used to facilitate purification of successfully engineered exosomes. It is used to target fluc specifically into exosomes via coiled-coil fused RNA binding proteins. The HiBit peptide is used for detection via luminescence.	+	This plasmid-encoded for CD63 fused to the HiBiT peptide and P9SN. The 6xHis tag inserted into the large extracellular loop of CD63 is used to facilitate purification of successfully engineered exosomes. It is used to target fluc specifically into exosomes via coiled-coil fused RNA binding proteins. The HiBit peptide is used for detection via luminescence.