Difference between revisions of "Part:BBa K2947002"
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<partinfo>BBa_K2947002 short</partinfo> | <partinfo>BBa_K2947002 short</partinfo> | ||
| − | d35S refers to Double GoldenGate compatible | + | d35S refers to Double GoldenGate compatible 35S Promoter. 35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus. |
We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate. | We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:34, 13 October 2019
d35S
d35S refers to Double GoldenGate compatible 35S Promoter. 35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus. We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]