Difference between revisions of "Part:BBa K2923009"

 
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===Usage and Biology===
 
===Usage and Biology===
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This LexA WT sequence correspond to LexA WT from E. coli strain JL1434 [Dimitrova, M., Younès-Cauet, G., Oertel-Buchheit, P., Porte, D., Schnarr, M., and Granger-Schnarr, M. (1998). A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli. Molecular and General Genetics MGG 257, 205–212.]
  
 
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Revision as of 12:10, 13 October 2019


LexA WT repressor

LexA (22.7 kDa) is a transcriptional repressor involved in SOS-system (a DNA damage response) by its binding with a promoter sequence of SOS-response regulatory genes. This LexA repressor sequence was previously described in Dimitrova et al. 1998 as part of the new LexA-based genetic system for monitoring and analyzing protein heterodimerization. The detection process involves two fusions proteins, which are two versions of chimeric LexA dimer. This sequence corresponds to wild type LexA repressor, recognizing LexA operator sequence CTGT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]