Difference between revisions of "Part:BBa K2984005:Design"
 
Line 12: Line 12:
  
 
===Source===
 
===Source===
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+
Inspired by the backbone “pICH47732” created by Sylvestre Marillonnet (Weber et al., 2011)[1]
  
 
===References===
 
===References===
 +
Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S (2011) A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLOS ONE 6(2): e16765. https://doi.org/10.1371/journal.pone.0016765

Latest revision as of 11:05, 13 October 2019


Level 1 b backbone with GoldenGate Fusionsite


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 729
    Illegal XhoI site found at 721
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1823
    Illegal BsaI.rc site found at 752


Design Notes

The codon usage optimization had to be adjusted to Chlamydomonas reinhardtii.


Source

Inspired by the backbone “pICH47732” created by Sylvestre Marillonnet (Weber et al., 2011)[1]

References

Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S (2011) A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLOS ONE 6(2): e16765. https://doi.org/10.1371/journal.pone.0016765