Difference between revisions of "Part:BBa K3142000"

 
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<partinfo>BBa_K3142000 short</partinfo>
 
<partinfo>BBa_K3142000 short</partinfo>
  
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what it is&#65306;The secretory signal peptide of the secreted protein usp45 gene of Lactococcus lactis MG1363 and the coding sequence of the first 11 amino acids of the mature peptide.
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The rcfB promoter, a member of the CRP-FNR positive regulation family, was amplified from chromosomal DNA lactis subsp. lactis IL1403. It is an important part of the P170 promoter regulated by acid. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated.
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what it does&#65306;Effectively secretes proteins from lactic acid bacteria to the outside of the cell.
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Revision as of 11:05, 13 October 2019


Acid-induced promoter（PrcfB）

The rcfB promoter, a member of the CRP-FNR positive regulation family, was amplified from chromosomal DNA lactis subsp. lactis IL1403. It is an important part of the P170 promoter regulated by acid. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
  • 1000
    COMPATIBLE WITH RFC[1000]