Difference between revisions of "Part:BBa K2923001"

Revision as of 10:47, 13 October 2019

Theophylline actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) requires the absence of ligand.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand).
 Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine.
-Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.
+Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) requires the absence of ligand.