Difference between revisions of "Part:BBa K3002017"
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This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. <a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody.
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This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. <a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))), a CDS and terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein can be detected via HA-antibody.
  
 
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Revision as of 10:40, 13 October 2019


3xHA tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))), a CDS and terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein can be detected via HA-antibody.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]