Difference between revisions of "Part:BBa K3002017"
Line 5: | Line 5: | ||
<html> | <html> | ||
− | This basic part contains the sequence of the | + | This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. <a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody. |
</html> | </html> |
Revision as of 10:32, 13 October 2019
3xHA tag for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]