Difference between revisions of "Part:BBa K1141000"
Line 9: Line 9:
 
<p align="center" id="legend">Figure 1: Excitation (left peak) and Emission (right peak) spectra for mCherry.</p>
 
<p align="center" id="legend">Figure 1: Excitation (left peak) and Emission (right peak) spectra for mCherry.</p>
  
−
===Translation Enhancing 5'-UTR (Improvement by iGEM Darmstadt 2019)===
+
<h1> Translation Enhancing 5'-UTR (Improvement by iGEM Darmstadt 2019) </h1>
  
 
</html>
 
</html>

Revision as of 07:32, 13 October 2019

Plac-RBS-mCherry-double terminator (IPTG-inducible)

This part contains all the necessary DNA to produce the red fluorescent protein mCherry with the PLac promoter. PLac repression is low in most E. coli strains and the promoter will be leaky. Efficient repression can be implemented by additionally transforming a strain with the pREP4 plasmid from the M15 E. coli strain of QIAGEN, or transforming the part into this strain. The figure below shows the excitation and emission spectra as can be obtained from CHROMA's spectra viewer:

Excitation-Emission spectra for mCherry

Figure 1: Excitation (left peak) and Emission (right peak) spectra for mCherry.

Translation Enhancing 5'-UTR (Improvement by iGEM Darmstadt 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]