Difference between revisions of "Part:BBa K3205001"

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<partinfo>BBa_K3205001 short</partinfo>
 
<partinfo>BBa_K3205001 short</partinfo>
  
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AreR is the regulatory protein of Acinetobacter sp. Strain ADP1. "are" operon and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
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Pare is a AreR-regulated σ54-dependent promoter in Acinetobacter sp. Strain ADP1 and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream genes transcription. This protein can form a complete gene expression regulator with the Pare promoter.
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AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol or other aromatic compounds. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. Combining this element with the AreR gene can form a complete gene expression regulation device for signaling pathways requiring benzyl alcohol induction.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 01:44, 13 October 2019


Pare promoter region

Pare is a AreR-regulated σ54-dependent promoter in Acinetobacter sp. Strain ADP1 and can be activated by benzyl alcohol or other aromatic compounds such as benzyl acetate, benzaldehyde, 2- and 4-hydroxybenzyl acetates, benzyl propionate and butyrate.


Usage and Biology

AreR is a member of the NtrC/XylR regulatory protein family and controls the expression of the areCBA genes in Acinetobacter sp. Strain ADP1. This σ54-type enhancer protein can bind to the repeat sequence upstream of the Pare promoter after activated by benzyl alcohol or other aromatic compounds. In the case of cofactor-induced DNA looping, a closed promoter complex will be formed with RNA polymerase to turn on the downstream gene transcription. Combining this element with the AreR gene can form a complete gene expression regulation device for signaling pathways requiring benzyl alcohol induction. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

For more details, please click here https://parts.igem.org/Part:BBa_K3205000