Difference between revisions of "Part:BBa K2983053:Design"
TomZaplana (Talk | contribs) |
|||
Line 1: | Line 1: | ||
+ | __NOTOC__ | ||
+ | <partinfo>BBa_K2983053 short</partinfo> | ||
+ | |||
+ | <partinfo>BBa_K2983053 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | Derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of ''Yarrowia lipolytica'' W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control. | ||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | DNA synthesis | ||
+ | |||
+ | ===References=== |
Latest revision as of 22:30, 12 October 2019
pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 192
Illegal PstI site found at 157 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.
Source
DNA synthesis