Difference between revisions of "Part:BBa K2983053:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K2983053 short</partinfo>
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<partinfo>BBa_K2983053 SequenceAndFeatures</partinfo>
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===Design Notes===
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Derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of ''Yarrowia lipolytica'' W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.
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===Source===
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DNA synthesis
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===References===

Latest revision as of 22:30, 12 October 2019


pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1). Four additional nucleotides (CACA) are present at the end of this part in order to be directly upstream of the ATG of a gene placed under its control.


Source

DNA synthesis

References