Difference between revisions of "Part:BBa K2984006"
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=='''Introduction'''==
 
=='''Introduction'''==
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Paromomycin belongs to a group of aminoglycoside antibiotics such as neomycin or dibekacin. These aminoglycosides are capable of inhibiting the eukaryotic translation, by binding within the large and small subunit of the 80S ribosome. This property allows paromomycin to be used as a selection marker. The paromomycin resistance BBa_K2703008, registered by the Cambridge team 2016, works by allowing the expression of an enzyme which catalyses the transfer of the gamma-phosphate of ATP to the hydroxyl group in 3’ position of the paromomycin molecule and allows the carrier of the gene to develop a resistance to paromomycin. Some organisms exhibit a higher expression of transgene enzymes when the genetic code is codon optimized for the specific organism. One of these organisms is the alga Chlamydomonas reinhardtii. With the following part, we propose a codon optimized version of the paromomycin resistance for use in Chlamydomonas reinhardtii. This part is optimal for paromomycin screening when using C. reinhardtii
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For the gold medal criterium of improving an existing part, we submit this codon optimized version of the paromomycin resistance registered as BBa K2703008.
  
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<h3> 1- Biological background </h3>
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Paromomycin belongs to a group of aminoglycoside antibiotics such as neomycin or dibekacin. These aminoglycosides are capable of inhibiting the eukaryotic translation, by binding within the large and small subunit of the 80S ribosome. This property allows paromomycin to be used as a selection marker. The paromomycin resistance BBa_K2703008, registered by the Cambridge team 2016, works by allowing the expression of an enzyme which catalyses the transfer of the gamma-phosphate of ATP to the hydroxyl group in 3’ position of the paromomycin molecule and allows the carrier of the gene to develop a resistance to paromomycin. Some organisms exhibit a higher expression of transgene enzymes when the genetic code is codon optimized for the specific organism. One of these organisms is the alga Chlamydomonas reinhardtii. With the following part, we propose a codon optimized version of the paromomycin resistance for use in Chlamydomonas reinhardtii. This part is optimal for paromomycin screening when using C. reinhardtii
  
 
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Revision as of 22:05, 12 October 2019


Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 298
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

For the gold medal criterium of improving an existing part, we submit this codon optimized version of the paromomycin resistance registered as BBa K2703008.

1- Biological background

Paromomycin belongs to a group of aminoglycoside antibiotics such as neomycin or dibekacin. These aminoglycosides are capable of inhibiting the eukaryotic translation, by binding within the large and small subunit of the 80S ribosome. This property allows paromomycin to be used as a selection marker. The paromomycin resistance BBa_K2703008, registered by the Cambridge team 2016, works by allowing the expression of an enzyme which catalyses the transfer of the gamma-phosphate of ATP to the hydroxyl group in 3’ position of the paromomycin molecule and allows the carrier of the gene to develop a resistance to paromomycin. Some organisms exhibit a higher expression of transgene enzymes when the genetic code is codon optimized for the specific organism. One of these organisms is the alga Chlamydomonas reinhardtii. With the following part, we propose a codon optimized version of the paromomycin resistance for use in Chlamydomonas reinhardtii. This part is optimal for paromomycin screening when using C. reinhardtii