Difference between revisions of "Part:BBa K2923001"
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Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. | Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. | ||
Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence. | Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence. | ||
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| + | The no highlighted sequences correspond to the riboswitch module. | ||
Revision as of 21:47, 12 October 2019
Theophylline actif aptazyme
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. Aptazyme is composed in 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) is related to ligand absence.
The no highlighted sequences correspond to the riboswitch module.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]