Difference between revisions of "Part:BBa K3002000"

 
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This basic part contains the coding sequence of a spectinomycin resistance and was built as a part of the Kaiser Collection. Combined with a promoter and a terminator, this level 0 construct mediates resistance to spectinomycin.
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This basic part contains the coding sequence of a spectinomycin resistance (B3-B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. <a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a> (PAR promoter (A1-A3)), <a href="https://parts.igem.org/Part:BBa_K3002034">BBa_K3002034</a> (Tub2 promoter (A1-A3)), or <a href="https://parts.igem.org/Part:BBa_K3002036">BBa_K3002036</a> (PSAD promoter (A1-A3))) and a terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)), this level 0 construct mediates resistance to spectinomycin.
 
It is used as a selection marker in Chlamydomonas and is, therefore, a mandatory component of every level 2 construct.  
 
It is used as a selection marker in Chlamydomonas and is, therefore, a mandatory component of every level 2 construct.  
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Revision as of 21:33, 12 October 2019


Spectinomycin Resistance for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of a spectinomycin resistance (B3-B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. BBa_K3002003 (PAR promoter (A1-A3)), BBa_K3002034 (Tub2 promoter (A1-A3)), or BBa_K3002036 (PSAD promoter (A1-A3))) and a terminator (e.g. BBa_K3002006 (RPL23 terminator)), this level 0 construct mediates resistance to spectinomycin. It is used as a selection marker in Chlamydomonas and is, therefore, a mandatory component of every level 2 construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 586
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]