Difference between revisions of "Part:BBa K3037000:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Followed by the biobrick prefix there is an RBS with an NgoIV restriction site, which is used for the RFC 25 standard when trying to make fusion proteins.
+
This BioBrick was site mutated to take out a XbaI restriction site
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Synthesized by Integrated DNA Technologies (IDT)
+
 
  
 
===References===
 
===References===

Latest revision as of 18:29, 12 October 2019


pOCC97 plasmid backbone for expression (optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5283
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5283
    Illegal BglII site found at 5169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5283
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5283
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5298
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 342
    Illegal NgoMIV site found at 3389
    Illegal NgoMIV site found at 3549
    Illegal NgoMIV site found at 5137
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2468


Design Notes

This BioBrick was site mutated to take out a XbaI restriction site


Source

References