Difference between revisions of "Part:BBa K3102009:Design"

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===References===
 
===References===
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"Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism." Chen Y.Y., Ko T.P., Chen W.H., Lo L.P., Lin C.H., Wang A.H. J. Struct. Biol. 169:25-35(2010)
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Chen Y.Y. et al., "Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism.", J. Struct. Biol. 169:25-35(2010)

Latest revision as of 16:59, 12 October 2019


GND (6-phosphogluconate dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 184


Design Notes

Sequence synthesized and optimized for E.coli on Geneius



Source

Corynebacterium glutamicum, sequence found through GenBank https://biocyc.org/gene?orgid=CORYNE&id=G18NG-11035-MONOMER#tab=TU


References

Chen Y.Y. et al., "Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism.", J. Struct. Biol. 169:25-35(2010)