Difference between revisions of "Part:BBa K1033910"

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===Characterization: NWU-China 2019===
 
===Characterization: NWU-China 2019===
  
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<partinfo>BBa_K1033910 AddReview 3</partinfo>
 
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<I>NWU-China 2019</I>
 
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The NWU-China team overexpressed fwYellow protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).
 
The NWU-China team overexpressed fwYellow protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).
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https://2019.igem.org/wiki/images/4/43/T--NWU-China--characterization-BBa_K1033910%28fwyellow%29.png
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[[File:T--NWU-China--characterization-BBa_K1033910%28fwyellow%29.png|900px|thumb|none|alt=fwYellow spectum.|Figure 1. fwYellow absorbance spectrum]]
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[[File:T--NWU-China--characterization-BBa_K1033910%28fwyellow%29.png|900px|thumb|none|alt=fwYellow spectum.|Figure 1. fwYellow absorbance spectrum]]
 
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Revision as of 13:30, 12 October 2019

fwYellow, yellow chromoprotein

This chromoprotein (also known as FezziwigYFP) naturally exhibits strong color when expressed. Compared to many other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-64 hours of incubation.

Usage and Biology

This part is useful as a reporter.

Uppsala2013 chromoproteins.JPG

iGEM13_Uppsala: Expression of chromoproteins. The images above show E coli constitutively expressing meffRed BBa_K1033922, aeBlue BBa_K1033929, tsPurple BBa_K1033905, asPink (asCP) BBa_K1033927, fwYellow BBa_K1033910, meffBlue BBa_K1033902, meffRed BBa_K1033922 and scOrange BBa_K1033913.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 109
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 486



Characterization: NWU-China 2019

The NWU-China team overexpressed fwYellow protein in E. coli and extracted and purified. The maximum absorbance was measured in a 96-well plate using a microplate reader (Synergy 2).

fwYellow spectum.
Figure 1. fwYellow absorbance spectrum