Difference between revisions of "Part:BBa K3102021:Design"
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===Design Notes== | ===Design Notes== | ||
| − | C1563T because Esp3I GGA | + | |
| + | We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. | ||
===Source=== | ===Source=== | ||
This sequence comes from E.coli and was found through Biocyc: | This sequence comes from E.coli and was found through Biocyc: | ||
| − | |||
https://biocyc.org/gene?orgid=ECOLI&id=EG11448 | https://biocyc.org/gene?orgid=ECOLI&id=EG11448 | ||
===References=== | ===References=== | ||
Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36. | Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36. | ||
| + | |||
Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86. | Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86. | ||
Latest revision as of 11:00, 12 October 2019
ACS (Acetyl-coenzyme A synthetase) Escherichia.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 373
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 802
- 1000COMPATIBLE WITH RFC[1000]
=Design Notes
We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
Source
This sequence comes from E.coli and was found through Biocyc: https://biocyc.org/gene?orgid=ECOLI&id=EG11448
References
Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.
Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.