Difference between revisions of "Part:BBa K3036005"
Line 4: | Line 4: | ||
This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2]. | This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2]. | ||
+ | |||
+ | Biology and Usage | ||
+ | |||
+ | This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells. [2] | ||
+ | |||
+ | <div style="text-align:center"> | ||
+ | <table border="solid" width="500px" height="150px" cellspacing="0" cellpadding="10" frame="solid" rules="solid" style="margin: auto"> | ||
+ | <tr align="center" valign="center" bgcolor="CCFFFF" > | ||
+ | <td colspan="2"><font size="3"><b>Arabinose-induced suicide switch</b></font></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><font size="2"><b>Function</b></font></td> | ||
+ | <td>Arabinose-induced suicide switch</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><font size="2"><b>Use in</b></font></td> | ||
+ | <td>Prokaryotes</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><font size="2"><b>RFC standard</b></font></td> | ||
+ | <td>RFC10 compatible</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><font size="2"><b>Backbone</b></font></td> | ||
+ | <td>pSB1C3</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><font size="2"><b>Derived from</b></font></td> | ||
+ | <td>Escherichia. coli DH5alpha </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 10:23, 12 October 2019
Arabinose-induced suicide switch
This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2].
Biology and Usage
This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells. [2]
Arabinose-induced suicide switch | |
Function | Arabinose-induced suicide switch |
Use in | Prokaryotes |
RFC standard | RFC10 compatible |
Backbone | pSB1C3 |
Derived from | Escherichia. coli DH5alpha |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]