Difference between revisions of "Part:BBa K3036005"

 
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This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2].
 
This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2].
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Biology and Usage
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 +
This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells. [2]
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<div style="text-align:center">
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<table border="solid"  width="500px" height="150px" cellspacing="0" cellpadding="10" frame="solid" rules="solid" style="margin: auto">
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<tr align="center" valign="center" bgcolor="CCFFFF" >
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<td colspan="2"><font size="3"><b>Arabinose-induced suicide switch</b></font></td>
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</tr>
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<tr>
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<td><font size="2"><b>Function</b></font></td>
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<td>Arabinose-induced suicide switch</td>
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</tr>
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<tr>
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<td><font size="2"><b>Use in</b></font></td>
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<td>Prokaryotes</td>
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</tr>
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<tr>
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<td><font size="2"><b>RFC standard</b></font></td>
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<td>RFC10 compatible</td>
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</tr>
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<tr>
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<td><font size="2"><b>Backbone</b></font></td>
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<td>pSB1C3</td>
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</tr>
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<tr>
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<td><font size="2"><b>Derived from</b></font></td>
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<td>Escherichia. coli DH5alpha </td>
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</tr>
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</table>
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</div>
  
 
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Revision as of 10:23, 12 October 2019


Arabinose-induced suicide switch

This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2].

Biology and Usage

This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells. [2]

Arabinose-induced suicide switch
Function Arabinose-induced suicide switch
Use in Prokaryotes
RFC standard RFC10 compatible
Backbone pSB1C3
Derived from Escherichia. coli DH5alpha

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]