Difference between revisions of "Part:BBa K2938008:Design"

 
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
His-tag fused to C- terminal  
 
His-tag fused to C- terminal  
 +
P20 was optimized for E.Coli
  
  
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
Gibson assembly
+
Gibson assembly, Tag added through primers
 +
P20 was Isolated from pVE4-ADRC [1]plasmid by PCR.
  
 
===References===
 
===References===
 +
[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.
 +
 +
[2] Y. Xu, M. Nagai, M. Bagdasarian, T. W. Smith, and E. D. Walker, “Expression of the p20 Gene from Bacillus thuringiensis H-14 Increases Cry11A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria,” Appl. Environ. Microbiol., vol. 67, no. 7, pp. 3010–3015, Jul. 2001.

Latest revision as of 09:48, 12 October 2019


P20 with His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag fused to C- terminal P20 was optimized for E.Coli


Source

Gibson assembly, Tag added through primers P20 was Isolated from pVE4-ADRC [1]plasmid by PCR.

References

[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.

[2] Y. Xu, M. Nagai, M. Bagdasarian, T. W. Smith, and E. D. Walker, “Expression of the p20 Gene from Bacillus thuringiensis H-14 Increases Cry11A Toxin Production and Enhances Mosquito-Larvicidal Activity in Recombinant Gram-Negative Bacteria,” Appl. Environ. Microbiol., vol. 67, no. 7, pp. 3010–3015, Jul. 2001.