Difference between revisions of "Part:BBa K2938007:Design"
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===Source=== | ===Source=== | ||
| − | Gibson assembly | + | Gibson assembly. Tag was added through primers. |
| + | Cyt1Aa was isolated from pVE4-ADRC plasmid by PCR. | ||
| + | and was optimized for E.Coli | ||
===References=== | ===References=== | ||
| + | [1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015. | ||
| + | |||
| + | [2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014. | ||
| + | |||
| + | [3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014. | ||
Latest revision as of 09:37, 12 October 2019
Cyt1Aa With Strep Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 419
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 419
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 717
Illegal BamHI site found at 96 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 419
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 419
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Strep-tag fused to C- terminal
Source
Gibson assembly. Tag was added through primers. Cyt1Aa was isolated from pVE4-ADRC plasmid by PCR. and was optimized for E.Coli
References
[1] V. Khasdan, “Thesis submitted in partial fulfillment : Cloning Combinations of Four Genes from Bacillus thuringiensis,” no. October, 2015.
[2] C. Xu, B. C. Wang, Z. Yu, and M. Sun, “Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins,” Toxins, vol. 6, no. 9. pp. 2732–2770, 01-Sep-2014.
[3] E. Ben-Dov, “Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.,” Toxins (Basel)., vol. 6, no. 4, pp. 1222–43, Mar. 2014.