Difference between revisions of "Part:BBa K2978003"
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This part makes reference to an endolysin from a bacteriophage that infects <i>Clostridium difficile</i>. It is a segment of the complete protein CD27L, which residues from 2 to 143 are homolougous to the domain <i>N-acetyl-muramoyl-L-alanine</i> amidase. This part codes for residues 1 to 179 of the mentioned protein. It has been codon optimized for <i>E.coli</i>. In previous works this domain has shown a faster lysis to different strains of the pathogen and a high level of selectivity against clostridia compared to the complete protein (Wang <i>et al.</i>, 2015). | This part makes reference to an endolysin from a bacteriophage that infects <i>Clostridium difficile</i>. It is a segment of the complete protein CD27L, which residues from 2 to 143 are homolougous to the domain <i>N-acetyl-muramoyl-L-alanine</i> amidase. This part codes for residues 1 to 179 of the mentioned protein. It has been codon optimized for <i>E.coli</i>. In previous works this domain has shown a faster lysis to different strains of the pathogen and a high level of selectivity against clostridia compared to the complete protein (Wang <i>et al.</i>, 2015). | ||
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| − | This basic part was characterized in the Composite [[Part:BBa_K2978000]] | + | This basic part was characterized in the Composite Part [[Part:BBa_K2978000 | BBa_K2978000]] |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 05:22, 12 October 2019
Causes cell lysis of the pathogen Clostridium difficile
This part makes reference to an endolysin from a bacteriophage that infects Clostridium difficile. It is a segment of the complete protein CD27L, which residues from 2 to 143 are homolougous to the domain N-acetyl-muramoyl-L-alanine amidase. This part codes for residues 1 to 179 of the mentioned protein. It has been codon optimized for E.coli. In previous works this domain has shown a faster lysis to different strains of the pathogen and a high level of selectivity against clostridia compared to the complete protein (Wang et al., 2015).
This basic part was characterized in the Composite Part BBa_K2978000
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]