Difference between revisions of "Part:BBa K3105677:Design"
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Revision as of 22:01, 11 October 2019
Expression construct HRP-2A-eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2086
Illegal XhoI site found at 1183 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2167
Illegal AgeI site found at 3176 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
AOX1 promoter inducible with methanol was used to achieve high expression as well as tight control of the expression system. α-factor secretion signal was used due to
Source
See basic parts