Difference between revisions of "Part:BBa K3128033:Experience"
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===Applications of BBa_K3128033=== | ===Applications of BBa_K3128033=== | ||
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| + | <'''Click-iT ™ Alexa Fluor ™ 488 sDIBO Alkyne confirmation:'''> | ||
| + | Click-iT ™ Alexa Fluor ™ 488 sDIBO alkyne was used to confirm whether Ompx is in memebrane and if the unnatural amino acid is incorporated into OmpX. This fluorophore is used to check the reaction of the click. If the unnatural amino acid is present, the fluorophore should "click" on the OmpX transmembrane protein and remain there. This can be analyzed with fluorescence microscopy | ||
| + | Here are the results obtained on unprocessed BL21 (1) and the results obtained for BL21 cotransformed with the 2 vectors (2). | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 20:21, 11 October 2019
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3128033
<Click-iT ™ Alexa Fluor ™ 488 sDIBO Alkyne confirmation:> Click-iT ™ Alexa Fluor ™ 488 sDIBO alkyne was used to confirm whether Ompx is in memebrane and if the unnatural amino acid is incorporated into OmpX. This fluorophore is used to check the reaction of the click. If the unnatural amino acid is present, the fluorophore should "click" on the OmpX transmembrane protein and remain there. This can be analyzed with fluorescence microscopy Here are the results obtained on unprocessed BL21 (1) and the results obtained for BL21 cotransformed with the 2 vectors (2).
User Reviews
UNIQ92e53e44caaa16c2-partinfo-00000000-QINU UNIQ92e53e44caaa16c2-partinfo-00000001-QINU