Difference between revisions of "Part:BBa K3038001"

 
Line 3: Line 3:
 
<partinfo>BBa_K3038001 short</partinfo>
 
<partinfo>BBa_K3038001 short</partinfo>
  
−
ADR : GenBank: P42327
 
−
ADH2_GEOSE              339 aa  (without the tags)
 
−
The RFC10 standard has been included in the design.
 
−
The sequence has been Designed with the geneious Software. C Myc and 6his Tags have been added to the Cterminal part of the ADR sequence.
 
  
−
[FUNCTION] Active with primary alcohols, including methanol.
+
===ADR : GenBank: P42327===
−
[CATALYTIC ACTIVITY] Reaction=a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH.
+
https://www.ncbi.nlm.nih.gov/protein/P42327
 +
===Alcohol dehydrogenase; Short=ADH or ADR===
 +
 
 +
===Thermophilic NAD+-dependent alcohol dehydrogenase===.
 +
Bears mainly an ethanol-dehydrogenase activity.
 +
 
 +
===Reaction===
 +
a secondary alcohol + NAD+ = a ketone + H+ + NADH
 +
EC:1.1.1.1
 +
 
 +
===Protein Sequence ===
 +
MVKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKSIKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALKVSGARPGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVNKKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFERMEKGKINGRIVLKLKEDEQKLISEEDLNSAVDHHHHHH
 +
 
 +
 
  
  

Revision as of 09:03, 11 October 2019


Alcohol dehydrogenase - Geobacillus stearothermophilus_Cmyc and 6His tags_Cterminal


ADR : GenBank: P42327

https://www.ncbi.nlm.nih.gov/protein/P42327

Alcohol dehydrogenase; Short=ADH or ADR

===Thermophilic NAD+-dependent alcohol dehydrogenase===. Bears mainly an ethanol-dehydrogenase activity.

Reaction

a secondary alcohol + NAD+ = a ketone + H+ + NADH EC:1.1.1.1

Protein Sequence

MVKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKSIKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALKVSGARPGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVNKKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFERMEKGKINGRIVLKLKEDEQKLISEEDLNSAVDHHHHHH



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]