Difference between revisions of "Part:BBa K3195003"
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This Cathepsin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria. | This Cathepsin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria. | ||
| − | + | ==Usage and Biology== | |
| − | + | Our BioBrick encodes a kind of cathepsin from Branchiostoma diverticulum epithelial cells. Cathepsin is a class of proteins found in the cells of various animal tissues, especially lysosome parts. Based on the results of bioinformatics analysis, the protein we found out belongs to cathepsin B. In Branchiostoma diverticulum epithelial cells, we speculate this kind of protein plays an important role in degrading cyanobacteria and microcystin. In fact, according to our experimental result, Cathepsin B protein has high efficiency in degradation of microcystin LR. So we construct a composite part so that other teams can easily use it. This BioBrick can be implemented in any host expression system by cloning it into an appropriate vector. | |
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===Characterization=== | ===Characterization=== | ||
Revision as of 07:36, 11 October 2019
His-tagged Cathepsin B
This Cathepsin B from Branchiostoma belcheri tsingtauense is originally a kind of hydrolase in lysosome.In our project,it has been used to biodegrade microcystin,a kind of biotoxin synthesized by cyanobacteria.
Usage and Biology
Our BioBrick encodes a kind of cathepsin from Branchiostoma diverticulum epithelial cells. Cathepsin is a class of proteins found in the cells of various animal tissues, especially lysosome parts. Based on the results of bioinformatics analysis, the protein we found out belongs to cathepsin B. In Branchiostoma diverticulum epithelial cells, we speculate this kind of protein plays an important role in degrading cyanobacteria and microcystin. In fact, according to our experimental result, Cathepsin B protein has high efficiency in degradation of microcystin LR. So we construct a composite part so that other teams can easily use it. This BioBrick can be implemented in any host expression system by cloning it into an appropriate vector.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 369
Illegal XhoI site found at 958 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]