Difference between revisions of "Part:BBa K2996001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | cas1 and cas2 indeed are seperated by one base pair, as it is in E.coli genome. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958. | 1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958. | ||
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| + | 2.Arslan Z , Hermanns V , Wurm R , et al. Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system[J]. Nucleic Acids Research, 2014, 42(12):7884-7893. | ||
Latest revision as of 00:08, 11 October 2019
cas1-cas2 complex
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 116
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 837
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
cas1 and cas2 indeed are seperated by one base pair, as it is in E.coli genome.
Source
The pRec plasmid containing cas1-cas2 is donated from Harris H. Wang from Columbia University. The cas1-cas2 cassette is amplified from NEB 10-beta. They are the only two Cas proteins found in all CRISPR–Cas systems, so cas1-cas2 are pretty conserved.
References
1.Sheth R U , Yim S S , Wu F L , et al. Multiplex recording of cellular events over time on CRISPR biological tape[J]. Science, 2017:eaao0958.
2.Arslan Z , Hermanns V , Wurm R , et al. Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system[J]. Nucleic Acids Research, 2014, 42(12):7884-7893.