Difference between revisions of "Part:BBa K3038000"
Line 3: | Line 3: | ||
<partinfo>BBa_K3038000 short</partinfo> | <partinfo>BBa_K3038000 short</partinfo> | ||
− | ADR : GenBank: P42327 | + | ===ADR : GenBank: P42327=== |
− | Alcohol dehydrogenase; Short=ADH | + | https://www.ncbi.nlm.nih.gov/protein/P42327 |
+ | ===Alcohol dehydrogenase; Short=ADH=== | ||
Thermophilic NAD+-dependent alcohol dehydrogenase. Bears mainly an ethanol-dehydrogenase activity. | Thermophilic NAD+-dependent alcohol dehydrogenase. Bears mainly an ethanol-dehydrogenase activity. | ||
− | reaction :a secondary alcohol + NAD+ = a ketone + H+ + NADH | + | ===reaction=== :a secondary alcohol + NAD+ = a ketone + H+ + NADH |
EC:1.1.1.1 | EC:1.1.1.1 | ||
− | PROTEIN SEQUENCE | + | ===PROTEIN SEQUENCE === |
MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS | MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS | ||
IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK | IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK |
Revision as of 09:43, 10 October 2019
Alcohol dehydrogenase - Geobacillus stearothermophilus cMYC and 6His N-terminal tags
ADR : GenBank: P42327
https://www.ncbi.nlm.nih.gov/protein/P42327
Alcohol dehydrogenase; Short=ADH
Thermophilic NAD+-dependent alcohol dehydrogenase. Bears mainly an ethanol-dehydrogenase activity.
===reaction=== :a secondary alcohol + NAD+ = a ketone + H+ + NADH EC:1.1.1.1
PROTEIN SEQUENCE
MHHHHHHKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAAHGDWPIKPKLPLIPGHEGVGIVVEVAKGVKS IKVGDRVGIPWLYSACGECEYCLTGQETLCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALK VSGARPGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVKAIHDQVGGVHAAISVAVN KKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSVKGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFER MEKGKINGRIVLKLKED
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]