Difference between revisions of "Part:BBa K3038000:Experience"
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Expression of the CMYC-6HIS-ADR and ADR-CMYC-6HIS recombinant proteins | Expression of the CMYC-6HIS-ADR and ADR-CMYC-6HIS recombinant proteins | ||
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Revision as of 09:17, 10 October 2019
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Applications of BBa_K3038000
PCR amplification of the PCR products
Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
Plasmid construction
Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
Expression of the CMYC-6HIS-ADR and ADR-CMYC-6HIS recombinant proteins
Activity
User Reviews
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