Difference between revisions of "Part:BBa K2938015"

Revision as of 09:16, 10 October 2019

Cry11Aa (Strep tag) + deGFP Device

PR1 - RBS - Cry11Aa(Strep tag) - RBS - deGFP - T500 Terminator

This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.

The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1870
Illegal XbaI site found at 2116
Illegal SpeI site found at 982
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1870
Illegal NheI site found at 56
Illegal NheI site found at 105
Illegal SpeI site found at 982
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1870
Illegal XhoI site found at 2849
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1870
Illegal XbaI site found at 2116
Illegal SpeI site found at 982
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1870
Illegal XbaI site found at 2116
Illegal SpeI site found at 982
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 PR1 - RBS - Cry11Aa(Strep tag) - RBS - deGFP - T500 Terminator
+This plasmid was planned on a pBEST plasmid backbone. It contains larvicidal activity, as it containing toxic subunit of the bacillus thuringiensis israelensis (BTI) pBtoxis plasmid which is toxic to mosquitoes larva.
+The plasmid was constructed using gibson assembly, then was incorporated into E.coli BL21 and DH5a using Heat shock, and into serratia marcescens 274 using electroporation.
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