Difference between revisions of "Part:BBa K3038001:Experience"
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Electrophoresis gel photography following deposits of N-ter and C-ter ADR PCR products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is NEB 1 kb Plus DNA Ladder. | Electrophoresis gel photography following deposits of N-ter and C-ter ADR PCR products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is NEB 1 kb Plus DNA Ladder. | ||
Revision as of 08:52, 10 October 2019
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Applications of BBa_K3038001
Characterization of the ADR constructs:
File:PCR amplification ADR.jpg
Electrophoresis gel photography following deposits of N-ter and C-ter ADR PCR products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is NEB 1 kb Plus DNA Ladder.
File:Plasmid construction ADR.jpg
Modelization of the ligation between ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
User Reviews
UNIQ43bf41980868ce5e-partinfo-00000000-QINU UNIQ43bf41980868ce5e-partinfo-00000001-QINU