Difference between revisions of "Part:BBa K3038000:Experience"

Revision as of 08:47, 10 October 2019

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3038000

PCR amplification of the PCR products

File:T--Poitiers--PCR amplification ADR-tab3.jpg

Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.

Plasmid construction

[[https://2019.igem.org/File:T--Poitiers--plasmid_construction_ADR-tab3.jpg%7Cthumb%7Ccenter%7C Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette. ]]

Expression of the recombinant protein

Activity

User Reviews

UNIQa457e3da9f716e63-partinfo-00000000-QINU UNIQa457e3da9f716e63-partinfo-00000001-QINU

@@ Line 17: / Line 17: @@
 Plasmid construction
-[[Image:https://2019.igem.org/File:T--Poitiers--plasmid_construction_ADR-tab3.jpg|thumb|center| Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
+[[https://2019.igem.org/File:T--Poitiers--plasmid_construction_ADR-tab3.jpg|thumb|center| Design of ADR N-ter/pSB1A3 and ADR C-ter/pSB1A3 with Geneious software. This map shows the pBAD promoter and its terminator flanking the coding sequence of the ADR protein. Also present in N-ter or C-ter are 6-His and c-myc tag. Finally, in the plasmid is present and ampicillin resistance cassette.
 ]]