Difference between revisions of "Part:BBa K3038000:Experience"
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| − | [[Image:PCR_amplification_ADR-tab3.jpg|400px|thumb|right|. Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3. | + | [[Image:https://2019.igem.org/wiki/images/4/40/T--Poitiers--PCR_amplification_ADR-tab3.jpg|400px|thumb|right|. Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3. |
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Revision as of 08:34, 10 October 2019
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how you used this part and how it worked out.
Applications of BBa_K3038000
PCR amplification of the PCR products
File:Https://2019.igem.org/wiki/images/4/40/T--Poitiers--PCR amplification ADR-tab3.jpg
. Electrophoresis photography following deposits on agarose gel 0.8% of enzymatic digestion products. The migration was performed at 100 volts for 30 minutes in TAE 1X. The marker used during the migration is the NEB 1 kb Plus Ladder. Lane 1 corresponds to the marker, lane 2 to the digested N-ter,ADR lane 3 to the digested C-ter ADR and lane 4 to the digested plasmid pSB1A3.
Plasmid construction
Expression of the recombinant protein
Activity
User Reviews
UNIQb6df11857759bba9-partinfo-00000000-QINU UNIQb6df11857759bba9-partinfo-00000001-QINU