Difference between revisions of "Part:BBa K2967021"

 
 
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<partinfo>BBa_K2967021 short</partinfo>
 
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ytfE is induced by NO as part of the NsrR regulon.ytfE promoter (PytfE) are normally repressed by NsrR, a protein native to most 'E. coli' cells. When nitrate or nitric oxide is bound to NsrR, halting the repression and allowing the transcription of downstream genes. Reference:Partridge, J.D., et al., NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility. Mol Microbiol, 2009. 73(4): p. 680-94.
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The promoter PytfE is sensitive to nitrate and nitrite. When nitrate and nitrite enter E. coli, they are converted to nitric oxide. Nitric oxide binds to the repressor protein NsrR, which inactivates PytfE to inhibit transcription of downstream genes. Then the promoter PytfE will be activated to express the luciferase.
  
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'''reference'''
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[1]Justino, M.C., et al., Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters. FEMS Microbiol Lett, 2006. 257(2): p. 278-84.<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2967021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2967021 SequenceAndFeatures</partinfo>

Latest revision as of 06:53, 10 October 2019


PytfE promoter, responsive to nitrate, nitrite and nitric oxide

The promoter PytfE is sensitive to nitrate and nitrite. When nitrate and nitrite enter E. coli, they are converted to nitric oxide. Nitric oxide binds to the repressor protein NsrR, which inactivates PytfE to inhibit transcription of downstream genes. Then the promoter PytfE will be activated to express the luciferase.

reference

[1]Justino, M.C., et al., Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters. FEMS Microbiol Lett, 2006. 257(2): p. 278-84.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]