Difference between revisions of "Part:BBa K2967019"
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'''Figure 2. Expressing vector is transformed into BL21 strain.''' After induction of nitric oxide, the culture is incubated at 37℃; for 2 hours. Western blotting is demonstrated to show the expression level of hIL-10. In order to equate the total number of cells in each lane, dilution is made to keep each sample at the same OD and μL of each sample is uploaded to each well.
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'''Figure 2. Expressing vector is transformed into BL21 strain.''' After induction of nitric oxide, the culture is incubated at 37℃; for 2 hours. Western blotting demonstrats the expression level of hIL-10. In order to equate the total number of cells in each lane, dilution is made to keep each sample at the same OD and μL of each sample is uploaded to each well.
  
 
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Revision as of 06:48, 10 October 2019


The yeaR promoter will initiate the express of IL-10 when nitric oxide appear

In order to see whether the nitric oxide sensor will work when it comes to expressing a certain protein, we add the IL-10 downstream and demonstrated the western blotting to show the result. It has been proved that the expression level can be induced when adding nitric oxide.

800px-T--NEU_China--part--pyear-il10-1.png

Figure 1. Diagram of expressing vector in backbone pCDFDuet-1. Promoter yeaR is an nitric oxide inducible promoter. Gene hIL-10 is one of our target protein.

800px-T--NEU_China--part--pyear%2Bli10-2.png

Figure 2. Expressing vector is transformed into BL21 strain. After induction of nitric oxide, the culture is incubated at 37℃; for 2 hours. Western blotting demonstrats the expression level of hIL-10. In order to equate the total number of cells in each lane, dilution is made to keep each sample at the same OD and μL of each sample is uploaded to each well.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]