Difference between revisions of "Part:BBa K3102013:Design"

Latest revision as of 14:09, 9 October 2019

ACS Operon (acs, yfcH, actP)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 373
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 802
Illegal AgeI site found at 2393
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3667

Design Notes

We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme.

Source

This sequence comes from E.coli and was found through Biocyc: https://biocyc.org/ECOLI/sequence-delimited?chromosome=COLI-K12&object=EG11448&up=0&down=2160&right=4287371&left=4283253&type=true

References

Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-none
-C1563T because Esp3I enzyme that we used for our construct GGA (golden gate assembly)
+We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct.
-A2425G because 2425 and 2428 EcoRI
+We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme.
-C2428T
 ===Source===
@@ Line 19: / Line 18: @@
 ===References===
+Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55.