Difference between revisions of "Part:BBa K3036005"

Revision as of 12:22, 9 October 2019

Arabinose-induced suicide switch

This arabinose-induced suicide switch consists of pBAD (BBa_K206000) and mazF (BBa_K302033), aiming to enable users of this microbe to terminate engineered bacteria inside their intestines with L-arabinose whenever needed. This suicide switch does no harm to human and can be used by direct in-taking of inducer. PBAD (BBa_K206000) is applied for heterologous gene expression due to its advantages, including moderately high expression levels, induction by a low-cost and non-toxic monosaccharide L-arabinose and tight regulation of transcription, which is particularly significant to expressing toxins [1]. MazF is an endoribonuclease that cleaves RNAs at ACA sites and causes the death of microbe, mediates suicide of cells without causing lysis of bacterial cells[2].

Sequence and Features