Difference between revisions of "Part:BBa K3102008:Design"
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+ | "Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004) |
Revision as of 21:19, 7 October 2019
ZWF (Glucose-6-phosphate 1-dehydrogenase)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 775
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 775
Illegal NotI site found at 656 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1332
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 775
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 775
Illegal NgoMIV site found at 48
Illegal NgoMIV site found at 765 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence synthesized and optimized for E.coli on Geneius
Source
Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969
References
"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004)