Difference between revisions of "Part:BBa K3128026:Design"

 
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
 
+
T18 is from <i>bordetella pertussis</i><br>
−
pUT18-ZIP plasmid from Euromedex BACTH kit was used (containing the T18 subpart and the leucine zipper gene). OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates.
+
Ompx is from <i> Escherichia coli</i> <br>
 +
<i> pUT18-ZIP </i> plasmid from Euromedex BACTH kit was used (containing the T25 subpart and the leucine zipper gene).<br>
 +
OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates.
  
 
===References===
 
===References===

Revision as of 20:34, 7 October 2019


OmpX-WT fused with Leucine Zipper and T18 subpart of B.Pertussis AC under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 1418
    Illegal AgeI site found at 1224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 179


Design Notes

The signal peptide has a conformation enabling the protein addressing to the membrane. This sequence is cut after the translocation. If the OmpX gene is used without changes, the leucine zipper gene is present before the signal peptide, thus generating a cut in the fusion protein leucine-zipper-OmpX.


Source

T18 is from bordetella pertussis
Ompx is from Escherichia coli
pUT18-ZIP plasmid from Euromedex BACTH kit was used (containing the T25 subpart and the leucine zipper gene).
OmpX gene and GGS linker were synthesized by IDT because they were unavailable on iGEM plates.

References