Difference between revisions of "Part:BBa K2967026:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs.
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===Source===
 
===Source===

Revision as of 15:39, 7 October 2019


The yeaR promoter added an extra NsrRBSs-RBS-Luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 228
    Illegal NgoMIV site found at 1572
    Illegal NgoMIV site found at 1593
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1478


Design Notes

To reduce sensor basal background, we inserted an extra NsrR binding sequence (NsrRBS) downstream of PyeaR to create a ‘roadblocking’ effect. The distance between the two NsrRs , the sensor’s leakiness and sensitivitcan be adjusted. However, due to time limitation we only modified 47 bp between two NsrRBSs.

Source

papers

References