Difference between revisions of "Part:BBa K2986004"
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
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===References===
 
===References===
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Revision as of 07:26, 7 October 2019


hGluc

The luciferase from the marine copepod Gaussia princeps (Gluc) is the smallest (∼19.9-kDa) coelenterazine (CTZ)-utilizing luciferase known thus far. A humanized variant of Gluc (hGluc), which was codon optimized for expression in cultured mammalian cells, has been widely used as a reporter gene. Gaussia luciferase exhibits an activity up to 1,000-fold higher than to Renilla reniformis luciferase (Rluc), firefly luciferase (Fluc), or bacterial luciferases (LuxAB). The outstanding sensitivity of Gluc-based assays was previously demonstrated detecting as low as 10−18 mol purified Gluc or one eukaryotic cell transiently expressing Gluc.



Design Notes

how to use the hGluc to represent the cytokine expression.


Source

Snapgene

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 604