Difference between revisions of "Part:BBa K3034004"
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Figure 1 shows that when the expression of Lysep3-D8 was induced by the addition of IPTG at a final concentration of 0.5 mM, the difference in Abs600 between the experimental group (+IPTG) and the control group (-IPTG) was larger and larger. That is, the Lysep3-D8 protein inhibited the growth of Escherichia coli (BL21) (inhibition rate was about 30.7%).
 
Figure 1 shows that when the expression of Lysep3-D8 was induced by the addition of IPTG at a final concentration of 0.5 mM, the difference in Abs600 between the experimental group (+IPTG) and the control group (-IPTG) was larger and larger. That is, the Lysep3-D8 protein inhibited the growth of Escherichia coli (BL21) (inhibition rate was about 30.7%).
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3034000 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3034000 parameters</partinfo>
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Revision as of 09:02, 6 October 2019


The fusion protein is an enhanced lysin-> lysep3-D8

This lysin is formed by fusion of lysep3 and D8,which lyses bacteria from inside and outside the cell.The bactericidal spectrum of lysep3-D8 is broad, as it can lyse both gram-negative bacteria and gram-positive bacteria(14 E. coli strains, 3 P. aeruginosa strains, 1 Acinetobacter baumannii strain,and 1 Streptococcus strain).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]