Difference between revisions of "Part:BBa K3076400:Design"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K3076450 short</partinfo>
+
<partinfo>BBa_K3076400 short</partinfo>
  
−
<partinfo>BBa_K3076450 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3076400 SequenceAndFeatures</partinfo>
  
  
Line 11: Line 11:
  
  
−
===Source===
+
===References===
−
 
+
 
The genomic sequence of cusA gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: 598714..601857). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.
 
The genomic sequence of cusA gene was  extracted from NCBI (ACCESSION: NC_000913 REGION: 598714..601857). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.
−
 
−
===References===
 

Revision as of 15:12, 5 October 2019


dsDNA substrate with KanR gene for cusA knockout in E. coli by Lambda Red Recombineering


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


References

The genomic sequence of cusA gene was extracted from NCBI (ACCESSION: NC_000913 REGION: 598714..601857). The kanamycin resistance gene was extracted from the sequence of pET28a plasmid sequence. Double terminator was extracted from iGEM part registry (BBa_B0015) which is the most commonly used double terminator with high efficiency.