Difference between revisions of "Part:BBa K3111202"
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<partinfo>BBa_K3111202 short</partinfo> | <partinfo>BBa_K3111202 short</partinfo> | ||
| − | - | + | This part encodes DARPin929_mScarlet. The use of this part enabled us to study the binding and uptake of DARPin929 by conducting cell culture studies on SK-BR-3 HER2+ cells. The DARPin is flanked by red fluorescent protein mScarlet on the C-terminus which allowed visualisation of binding under confocal microscopy and a StrepII-tag downstream of this fusion, which allows for column-based purification of the expressed protein. It is expressed under a T7 promoter and a strong RBS. |
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<partinfo>BBa_K3111202 parameters</partinfo> | <partinfo>BBa_K3111202 parameters</partinfo> | ||
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| + | ==Experimental Results== | ||
Revision as of 15:08, 5 October 2019
DARPin929_mScarlet_StrepII
This part encodes DARPin929_mScarlet. The use of this part enabled us to study the binding and uptake of DARPin929 by conducting cell culture studies on SK-BR-3 HER2+ cells. The DARPin is flanked by red fluorescent protein mScarlet on the C-terminus which allowed visualisation of binding under confocal microscopy and a StrepII-tag downstream of this fusion, which allows for column-based purification of the expressed protein. It is expressed under a T7 promoter and a strong RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 575
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1147
- 1000COMPATIBLE WITH RFC[1000]