Difference between revisions of "Part:BBa K3111021"

 
(updating information)
 
Line 3: Line 3:
 
<partinfo>BBa_K3111021 short</partinfo>
 
<partinfo>BBa_K3111021 short</partinfo>
  
−
Fluorescent protein with excitation peak at 569 nm and emission peak at 594 nm
+
mScarlet is a red fluorescent protein designed by Bindels et al. in 2016 [1] with excitation peak at 569 nm and emission peak at 594 nm. This part was designed to be fused to N or C terminus of DARPin929 (<partinfo>BBa_K3111011</partinfo>), and as such has had its stop codon and start codons removed.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 18:
 
<partinfo>BBa_K3111021 parameters</partinfo>
 
<partinfo>BBa_K3111021 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074

Latest revision as of 12:55, 5 October 2019


mScarlet

mScarlet is a red fluorescent protein designed by Bindels et al. in 2016 [1] with excitation peak at 569 nm and emission peak at 594 nm. This part was designed to be fused to N or C terminus of DARPin929 (BBa_K3111011), and as such has had its stop codon and start codons removed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074